Figure 2.

Loss of NOD2 alters trajectories of recovery from antibiotic-induced body weight loss and bacterial dysbiosis in 20-week-old mice. The 20-week-old WT and Nod2-KO mice were treated with an antibiotic cocktail for 10 days and were followed up until day 50. (A) Percentage change in body weight of WT and Nod2-KO mice. The percentage body weight was calculated based on initial body weight at the time the experiment started. Data are represented as means ± SEM values (n = 5 WT, n = 5 Nod2-KO). Brown circles indicate collected fecal pellets. ∗Significant difference (P < .05) between the genotypes was evaluated using the Student t test. (B) PCoA using the Bray–Curtis dissimilarity of the fecal bacterial communities across time (days), where PC1 explains 25.0% and PC2 explains 15.6% of the data variance. Each dot represents the bacterial composition of an individual fecal sample. Samples are colored by day. Filled circles represent WT and open circles represent Nod2-KO mice. (C) Cumulative Bray–Curtis distance of samples travelled from d0 to d10, d20, d27 to d50. The distance travelled indicates the degree of change in bacterial composition over time during antibiotic perturbation and recovery. Note the decreased travelled distance in the Nod2-KO compared with the WT. (D) Relative abundance (%) of 16S bacterial community composition across all time points in feces of WT and Nod2-KO mice at genus level. AB, antibiotics; uncl, unclassified.