Fig. 11.

Effects of SPC25 on invasion, migration, and adhesion of HCC cells. (A) Scratch test showing that the knockdown of SPC25 inhibited the migration of HepG2 and SMMC7721 cells. (B) Transwell assays were performed 48 h after SPC25‐siRNA transfection. Comparison of the number of cells on the Transwell membrane shows that migration and invasion are significantly inhibited. (C) The adhesion between HepG2 and SMMC7721 cells and the matrix significantly decreased after SPC25 knockdown. (A) Scale bar: 200 μm. (B, C) Scale bar: 100 μm. *P < 0.05, **P < 0.01, and ***P < 0.001. All experiments were performed in triplicate, and data represent the mean ± SD; statistical comparisons were calculated using Student's two‐tailed t‐tests (versus control group).