Table 2.
Antibodies.
| Antibody | Dilution | Conjugate/ Source | Lot‐Nr. | Application |
|---|---|---|---|---|
| GAPDH | 1 : 1000 | Mouse | sc‐365062 | WB |
| DDR2 | 1 : 200 | Mouse | MAB25381 | ICC |
| Vimentin | 1 : 200 | Rabbit | #5741 | ICC |
| αSMA |
1 : 200 (ICC) 1 : 1.000 (WB) |
Mouse | A5228 | ICC/ WB |
| Collagen 1 | 1 : 200 | Rabbit | ab34710 | ICC |
| Collagen Iα1 | 1 : 100 | Goat | MyBioSource | ICC |
| SMAD2/3 | 1 : 1.000 | Rabbit | #3102 | WB |
| PhosphoSMAD2/3 | 1 : 1.000 | Rabbit | #8828 | WB |
| Anti‐mouse | 1 : 10 000 | Peroxidase | A3682 | WB |
| Anti‐rabbit | 1 : 10 000 | Peroxidase | 111‐035‐045 | WB |
| Alexa Fluor 546 (anti‐mouse) | 1 : 400 | Streptavidin | Z25004 | ICC |
| Alexa Fluor 546 (anti‐rabbit) | 1 : 400 | Streptavidin | Z25304 | ICC |
| Alexa Flour 555 (anti‐goat) | 1 : 500 | Rabbit | ab150146 | ICC |