Fig. 6.

Effects of Complex III inhibitor on HIF1α. ROS generation in Raw264.7 cells in response to treatment with antimycin A for 2h at the indicated concentrations. A, ROS generation was measured by Amplex Red assay (n = 3 independent experiments, in duplicate). Fluorescent values were normalized to cell number. Significance was calculated by one-way ANOVA with Tukey's multiple-comparison test, and data are presented as treatment group versus no-treatment control. In all experiments error bars represent standard deviations (*p < 0.05, **p < 0.01). B, Representative immunoblots of HIF1α, p38, phosphorylated p38, phosphorylated AMPKα proteins and GAPDH as loading control. C. Immunoblot analysis of Raw 264.7 cell extracts treated with indicated concentrations of AA for 2h. GAPDH was used as a loading control. The blots are representative of three different runs. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)