Fig. 2.

Measuring Mt function to determine the optimal treatment time. RPE cells were plated in a 96-well plate and incubated overnight. Cells were treated with compounds (NAC, Rapamycin, PQQ, or NMN) for 24 or 48 h. Mitochondrial function was evaluated using XFe96 Extracellular Flux Analyzer. An example trace of oxygen consumption rate (OCR) from a single donor treated with the specified drug is shown (left). Average calculated values (±StDev) of Mt function (compared to untreated controls) for two donors are shown (right). BR = basal respiration; MR = maximal respiration; SRC = spare respiratory capacity; ATP = ATP production.