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. 2020 Apr 20;34:101465. doi: 10.1016/j.redox.2020.101465

Fig. 4.

Fig. 4

Effect of UPS and endo-lysosomal pathway inhibition on human cortical neuron survival. (A) Shown are the confocal images of immunofluorescence against neuronal marker MAP2, excitatory marker VGLUT and the inhibitory marker VGAT in differentiated H1-ESCs. (B–E) After 24 h treatment with the indicated drugs, cell viability (B, D) and apoptosis (C, E) were measured using ApoTox-Glo triplex assay kit. Data presented are from 3 to 6 independent biological replicates. Scale bars are 20 μm. Error bars are SEM. **, p-value < 0.005; *, p-value < 0.05.