Fig. 1.

Sulfide induces NO release from hypoxic endothelial cells. HUVECs (1 × 10⁶ cells) were assessed for sulfide-mediated NO generation using enhanced chemiluminescence. Buffer was exposed to 25, 50, 100 μM of nitrite (A) or Sulfide (B) in the NOA reaction chamber to establish a normoxic cell baseline; or under hypoxia nitrite (C) or Sulfide (D). Quantitation of NO generation from cells exposed to 25 and 50 μM of Sulfide at various times of hypoxia (0h, 3h, 4h and 5h) (E) NO generation from cells exposed with 25, 50 and 100 μM of Sulfide at 4h normoxia or hypoxia (F). n = 6; *p = 0.000002, 0.000060 and 0.000183 respectively for panel E; *p<0.005 for panel F.