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NO generation is independent of NOS. Exposure of hypoxic cells (HUVECs; 4h) to Sulfide (100 μM) (A) or DATS (50 μM) (B). Exposure of hypoxic cells (HUVECs) to Sulfide (100 μM) and l-NAME (200 μM) (C) or DATS (100 μM) and l-NAME (200 μM) (D). Exposure of hypoxic cells (HUVECs) to Sulfide (100 μM) and Febx (10 nM) (E) or DATS (100 μM) and Febx (10 nM) (F). Average rate of NO production, quantitation from A-F for sulfide (G), *p = 0.001, and DATS (H), *p = 0.0005, where n = 6.
