Fig. 5.

Electron flux between the Mo-co and proximal Fe/S center is requisite for Sulfide and DATS-mediated effects. Schematic of electron flux in XOR reduction of nitrite (A). Purified XO (20 mU, dissolved in HBSS) plus NO₂^- (100 μM) were exposed to either DPI and/or DCPIP (after/before addition of DATS) and NO release was assessed (B) and quantified (C). The capacity for DATS to reduce the FAD of XOR was determined by following alterations in absorbance (λ = 450 nm) of purified XO in KPi pH 7.4 (D). n = 4; *p<0.001.