Figure 11.

Process of Collecting and Mapping Neurons from the Rat Heart

(A and B) (A) A rat heart was sectioned at 20 μm, imaged, and put into a 3D stack in TissueMaker. The red outline shows a specific section shown in (B).

(C–E) (C) The selected region from (B) is shown in a magnified view before and after laser capture microdissection, where the single neuron that has been collected can be seen on the LCM cap (middle). Scale bar: 50 μm. Distribution of mapped neurons (D) and sampled neurons (E) in the context of their 3D location as seen in TissueMapper.

(F) Normalized qRT-PCR data showing expression of 154 genes for the 151 samples collected.