Figure 7.

Identification of Neuronal Clusters in the Rat heart

Nine clusters of neurons were identified using the partitioning around medoids (PAM) algorithm, where identified clusters are shown in different colors.

(A) Visualization of mapped neurons in their 3D orientation in TissueMapper; contours show the aorta (orange) as well as the left atrium (green).

(B) Visualization of mapped neurons in their 3D orientation.

(C–E) Visualization of mapped neurons in their 3D orientation rotated to show different points of view.

(F) Flat-mount projection of mapped neurons where the height of the contours are proportional to the density of neurons. The orientation in (E) matches the flat-mount projection in (F). (See also Video S5). Scale bar: 500 μm.