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. 2020 Jun 24;9(6):bio053611. doi: 10.1242/bio.053611

Fig. 3.

Fig. 3.

Gata5 and gata6 are redundant for cardiac specification. ISH was performed using offspring from a gata5+/−, gata6+/− in-cross with probes targeting either myocardial marker myl7 (left panels) or cardiac progenitor marker nkx2.5 (right panels). (A) gata5−/−, gata6−/− mutants exhibit a heartless phenotype and a complete loss of cardiac progenitors (indicated by arrows). Representative scale bar in WT panels: 0.1 mm. (B) Quantification of nkx2.5 staining shown in A. Error bars represent s.e.m. *P<0.05, ****P<0.0001. (C) Schematic showing that loss of gata5 and 6 disrupts the initial stages of heart development.