Fig. 6.

Cardiac development depends on the dosage of gata4/5/6 alleles. ISH was performed using offspring from crossing gata4^+/−, gata5^+/−, gata6^+/− triple heterozygous adults and a probe targeting myocardial marker myl7. (A,A′) Triple heterozygous embryos display abnormal heart tube morphology compared to wild-type siblings, although they can survive to adulthood. Loss of a single gata5 or gata6 allele in a gata4^−/−, gata6^−/− (B,B′) or gata4^−/−, gata5^−/− (C,C′) background, respectively, results in a severe loss of cardiac tissue. Representative scale bar in WT panel: 0.1 mm.