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. 2020 Jun 22;147(12):dev178988. doi: 10.1242/dev.178988

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© 2020. Published by The Company of Biologists Ltd

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Fig. 6. — Notch1-deleted TECs are unable to contribute to the mTEC lineage. (A) Scheme for generating TECs with mosaic Notch1 deletion for analysis. Pregnant dams are injected at E8.5 (8 dpf), embryos are collected at E15.5 and the thymus dissected and dissociated into single cells. (B) Gating for isolation of EpCam⁺CD45⁻ TECs. (C) Gating for MHCII^lo and MHCII^hi cTEC (UEA-1⁻) and mTEC (UEA-1⁺). (D) PCR of genomic DNA with primers specific for the wild-type undeleted and deleted alleles of Notch1. Genotypes and cell populations represented are indicated above each lane.