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. 2020 Jun 22;147(12):dev184093. doi: 10.1242/dev.184093

Fig. 2.

Fig. 2.

An E2A forced homodimer drives Sox1 expression under self-renewal conditions. (A) Schematic representation of monomeric E2A and forced E2A homodimer constructs. (B) Immunostaining of inducible E2A forced homodimers and monomers cultured in LIF/serum+dox. Scale bars: 30 µm. (C) Quantification of immunostaining for E2A monomers and homodimers cultured in LIF/serum+dox. A range of 500 to 5000 cells were manually scored for each timepoint. (D) qRT-PCR analysis of dox-inducible overexpression of E2A monomers and homodimers in LIF/serum. Expression is normalised to day 0 minus dox controls. (E) qRT-PCR analysis of two mutant forced homodimer clones (C570A-1 and C570A-9) induced in LIF/serum+dox. Expression values are normalised to the housekeeping gene Sdha. Data are mean±s.d., n=3 biological replicates. *P<0.05, two-tailed paired Student's t-test.