Extended Data Figure 6. PET/CT and biochemical analysis of KL tumors.

a, Crystal structure of complex I (PDB: 5lc5) is shown with NDUFS1 and NDUFV1 subunits is red and FeS clusters in yellow. b-c, PET/CT images from three KL mice that were imaged on sequential days with ¹⁸FBnTP (top panel) and ¹⁸F-FDG (bottom panel). Tumors are circled, H – heart. Maximum uptake value for each tumor after normalization to maximum uptake of the heart is indicated. e, Western blot analysis from lung nodules that were isolated from mice imaged in b-d. Two lung tumors from mouse 5372 (imaged in b) are shown – T1 in blue (low ¹⁸F-FDG and Glut1 levels; high ¹⁸FBnTP and high Ndufs1 and Ndufv1 levels); and T2 in red (high ¹⁸F-FDG and Glut1 levels; low ¹⁸FBnTP and low Ndufs1 and Ndufv1 levels). Experiments in b-d are representative of three independent mouse experiments. Experiment in e was done once.