Fig. 2.

Effects of insulin on BMP content and FA composition. Mice were fasted overnight and given an intraperitoneal injection of vehicle (saline) or insulin (0.5 IU/kg), and tissues were collected after 2 h. A: Western blot analysis of phospho-AKT (pAKT, Ser437) and AKT (pan) as loading controls in tissue lysates and respective quantification upon (B) refeeding and (D) insulin treatment. C: Relative changes in total BMP content of tissues from refed mice (calculated from the data shown in Fig. 1) and (D) insulin-treated mice. Data are presented as means ± SDs. Statistical significance was evaluated by an unpaired two-tailed Student’s t-test (*P < 0.05, **P < 0.01, and ***P < 0.001; n = 5–8).