Fig. 2.

GDPD3 gene expression in mouse tissues. Twelve-week-old mice were fasted for 24 h (three male and three female) and tissues were harvested for measurement of Gdpd3 mRNA levels by real-time PCR (A). In a second study, 12-week-old male mice were fasted for 24 h (n = 5), and another group was fasted for 24 h followed by refeeding a standard chow diet for 12 h (n = 5); fasting times were staggered so that both groups of mice were euthanized together. Livers were collected to measure TAG levels (B) and Gdpd3 mRNA levels (C) by real-time PCR. All results are mean ± SEM and analyzed using a two-tailed Student’s unpaired t-test. Ct, cycle threshold; WAT, white adipose tissue; BAT, brown adipose tissue.