Fig. 3.
The effect of human GDPD3 expression on hepatic LysoPA content in chow-fed mice. Six-week-old male mice were treated with the indicated AAV (n = 6 per genotype). Eighteen weeks after AAV injection, livers were collected from chow-fed mice for the measurement of mouse Gdpd3 mRNA (A) and human GDPD3 protein (B); adipose and muscle tissues were also collected for human GDPD3 protein detection (C). D: Chow-fed mouse livers were also used for LysoPA measurement. Six to ten weeks after AAV injection (n = 2 per genotype), primary hepatocytes from chow-fed mice were isolated and incubated with [14C]palmitic acid for 0, 1, 2, and 4 h (n = 6 per time point). E: Newly synthesized [14C]LysoPA was measured as well as the expression of mouse Gdpd3 mRNA (F) and human GDPD3 protein (G). All results are mean ± SEM and analyzed using a two-tailed Student’s unpaired t-test (A, D, F) or two-way ANOVA with Sidak multiple comparisons (E). DPM, disintegrations per minute.