Fig. 5.

Overexpression of human GDPD3 in Western diet-fed mice increases hepatic lipid content. Six-week-old male mice were treated with the indicated AAV (n = 10 per genotype) for 2 weeks and then fed a Western diet for 12 weeks. Western diet-fed mice were used for the measurement of body weight at 8, 12, 16, and 20 weeks of age (A). Livers of Western diet-fed mice were collected for the measurement of wet weight (B), TAG (C), CE (D), FC (E), PL (F), and LysoPA (16:0) (G). Glucose tolerance test (H) and insulin tolerance test (I) were performed between 8 and 10 weeks of a Western diet feeding. All results are mean ± SEM and analyzed using a two-tailed Student’s unpaired t-test.