Fig. 6.

The effect of human GDPD3 overexpression on lipid synthesis in primary hepatocytes from Western diet-fed mice. Ten to twelve weeks after the indicated AAV injection (n = 2 per genotype), primary hepatocytes were isolated from Western diet-fed mice (8–10 weeks of feeding) and incubated with the indicated radiolabels for up to 180 min (n = 3 per time point). Uptake of [¹⁴C]palmitic acid (A), formation of [³H]TAG (B), [³H]CE (C), and [³H]PL (D) from [³H]oleic acid, de novo synthesis of [¹⁴C]TAG (E), [¹⁴C]CE (F), and [¹⁴C]PL (G) from [¹⁴C]acetic acid, as well as expression of human GDPD3 protein (H), were measured. All results are mean ± SEM and analyzed using two-way ANOVA with Sidak multiple comparisons (A–G). DPM, disintegrations per minute.