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Impact of apoptosis on the total cellular sphingolipid content in U251 cells. After STS treatment, lipid extraction and purification are performed from cell pellets. Sphingolipid levels are then measured by UPLC-MS. A: Cumulative amounts of all chain lengths are expressed in picomoles per 106 cells. B: Levels of specific C16 ceramide (picomoles per 106 cells) (mean ± SEM; *P < 0.05, **P < 0.01 vs. nontreated cells).
