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. 2020 Apr 29;61(7):1025–1037. doi: 10.1194/jlr.RA120000628

Fig. 6.

Fig. 6.

Activation of ASMase subforms in the MAM fraction in the U251 cell line. Subcellular fractionation is performed from STS-treated cells. ASMase activity present in 50 μl was measured in the MAM fraction. The graphs show the results from three independent experiments. The impact of STS-induced apoptosis on total ASMase (A), L-ASMase (B), S-ASMase (C), and NSMase (D) in the MAM fraction. Results are expressed in picomoles per microgram of proteins per hour (mean ± SEM; *P < 0.05, **P < 0.01 vs. nontreated cells). E: Western blot analysis of the fractions obtained in U251 cells for the copurification of Golgi (GM130) and lysosomes (LAMP2). The blots are representative of three independent experiments. F: Western blot analysis of U251 MFs during STS treatment. The blots are representative of three independent experiments.