TABLE 1.
Subcellular fractionation performed from mouse livers
| H | MF | PM | MAM | ER | Cyto | |||||||
| Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |
| Ceramide | ||||||||||||
| C14:0 | 4.87 | 1.61 | 7.11 | 1.25 | 9.10 | 3.96 | 4.72 | 1.49 | 10.47 | 2.07 | 0.51 | 0.37 |
| C16:1 | 1.25 | 0.58 | 1.98 | 0.28 | 3.72 | 1.10 | 2.57 | 0.60 | 1.43 | 0.41 | 0.85 | 0.42 |
| C16 | 38.76 | 12.63 | 78.41 | 10.11 | 112.00 | 34.92 | 58.63 | 17.82 | 97.24 | 13.90 | 3.51 | 0.52 |
| C18:1 | 0.93 | 0.31 | 2.03 | 0.27 | 3.84 | 1.07 | 0.29 | 0.29 | 1.13 | 0.29 | 0.19 | 0.19 |
| C18 | 32.37 | 7.77 | 137.45 | 28.70 | 144.17 | 22.86 | 204.34 | 56.83 | 54.82 | 8.11 | 152.20 | 34.72 |
| C20:1 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| C20 | 1.64 | 0.60 | 4.09 | 0.64 | 8.86 | 4.07 | 2.81 | 0.54 | 6.05 | 1.34 | 0.25 | 0.25 |
| C22:1 | 1.09 | 0.38 | 2.27 | 0.58 | 4.08 | 1.88 | 1.35 | 0.29 | 4.24 | 0.49 | 0.00 | 0.00 |
| C22 | 7.59 | 2.52 | 18.14 | 2.10 | 35.72 | 11.37 | 12.84 | 4.17 | 25.24 | 4.06 | 1.30 | 0.05 |
| C24:1 | 123.55 | 41.27 | 258.84 | 29.87 | 445.91 | 131.01 | 173.11 | 48.49 | 358.68 | 36.25 | 11.39 | 2.48 |
| C24 | 40.53 | 14.50 | 84.53 | 7.12 | 131.50 | 35.35 | 57.68 | 13.29 | 120.36 | 13.37 | 6.17 | 1.17 |
| SM | ||||||||||||
| C14:0 | 317.93 | 68.99 | 1,464.30 | 106.82 | 593.72 | 131.79 | 1608.66 | 64.32 | 993.28 | 135.61 | 21.61 | 10.82 |
| C16:1 | 376.22 | 81.26 | 1,708.56 | 97.20 | 672.38 | 123.04 | 1,852.92 | 28.60 | 1,135.45 | 158.04 | 36.65 | 3.90 |
| C16 | 4,653.71 | 880.03 | 22,298.32 | 2,219.49 | 9,495.85 | 1,969.88 | 24,336.04 | 714.99 | 13,682.74 | 1,602.26 | 906.61 | 389.91 |
| C18:1 | 112.37 | 25.73 | 470.80 | 45.54 | 192.90 | 24.54 | 513.99 | 23.53 | 307.04 | 24.20 | 6.50 | 3.58 |
| C18 | 422.00 | 79.76 | 2,019.36 | 88.37 | 981.44 | 183.30 | 2,030.76 | 31.16 | 1,152.21 | 137.07 | 119.18 | 73.24 |
| C20:1 | 18.76 | 5.59 | 74.43 | 10.07 | 35.55 | 3.01 | 65.03 | 8.90 | 51.85 | 8.20 | 0.00 | 0.00 |
| C20 | 81.09 | 19.07 | 406.08 | 35.36 | 157.10 | 22.46 | 382.61 | 39.81 | 223.89 | 32.96 | 57.25 | 54.12 |
| C22:1 | 116.03 | 18.77 | 529.34 | 34.78 | 224.42 | 28.59 | 543.24 | 31.37 | 315.91 | 46.55 | 19.85 | 14.53 |
| C22 | 204.01 | 40.39 | 1,108.36 | 132.84 | 475.91 | 96.14 | 1,014.94 | 141.00 | 551.23 | 78.40 | 22.84 | 13.53 |
| C24:1 | 1,656.56 | 257.77 | 8,205.66 | 1,135.30 | 3,683.24 | 932.79 | 8,478.13 | 442.03 | 4,878.83 | 917.29 | 385.63 | 170.81 |
| C24 | 371.86 | 53.23 | 2,049.34 | 333.96 | 898.11 | 235.67 | 1,839.27 | 232.73 | 1,096.05 | 221.38 | 368.50 | 323.24 |
| Sphingosine | ||||||||||||
| C18:1 | 21.66 | 4.01 | 113.35 | 18.81 | 213.25 | 27.40 | 128.50 | 71.59 | 23.56 | 5.10 | 61.79 | 18.04 |
| Sphinganine | ||||||||||||
| C18 | 2.31 | 0.56 | 12.18 | 1.86 | 34.56 | 1.96 | 5.13 | 2.24 | 2.32 | 0.35 | 1.53 | 0.48 |
| Glucosyl-ceramide | ||||||||||||
| C14:0 | 0.00 | 0.00 | 0.01 | 0.01 | 0.00 | 0.00 | 0.01 | 0.01 | 0.01 | 0.01 | 0.00 | 0.00 |
| C16:1 | 0.51 | 0.13 | 2.86 | 0.27 | 1.73 | 0.31 | 1.99 | 0.03 | 1.76 | 0.04 | 0.00 | 0.00 |
| C16 | 50.62 | 11.05 | 252.43 | 3.77 | 199.79 | 18.40 | 197.40 | 12.31 | 127.85 | 9.44 | 4.15 | 0.40 |
| C18:1 | 0.14 | 0.08 | 0.64 | 0.33 | 0.72 | 0.36 | 0.31 | 0.31 | 0.25 | 0.12 | 0.00 | 0.00 |
| C18 | 3.98 | 1.03 | 21.57 | 1.23 | 17.76 | 2.21 | 18.60 | 2.38 | 9.04 | 0.81 | 0.12 | 0.12 |
| C20:1 | 0.07 | 0.07 | 0.00 | 0.00 | 0.30 | 0.19 | 0.07 | 0.07 | 0.11 | 0.09 | 0.00 | 0.00 |
| C20 | 1.12 | 0.18 | 6.38 | 0.26 | 5.73 | 0.50 | 5.74 | 0.39 | 2.14 | 0.07 | 0.04 | 0.04 |
| C22:1 | 5.86 | 1.29 | 32.69 | 2.48 | 28.52 | 1.77 | 24.07 | 2.45 | 12.70 | 1.29 | 0.17 | 0.17 |
| C22 | 0.25 | 0.17 | 1.28 | 0.66 | 1.37 | 0.69 | 0.89 | 0.49 | 0.45 | 0.23 | 0.01 | 0.01 |
| C24:1 | 44.09 | 8.69 | 255.23 | 10.99 | 242.01 | 13.46 | 166.90 | 16.72 | 99.79 | 8.43 | 3.53 | 0.53 |
| C24 | 30.63 | 7.12 | 154.09 | 11.89 | 126.75 | 4.81 | 111.96 | 10.42 | 84.62 | 7.46 | 2.02 | 0.38 |
| Lactosyl-ceramide | ||||||||||||
| C14:0 | 1.13 | 0.55 | 4.83 | 1.51 | 1.72 | 0.51 | 4.31 | 0.90 | 3.51 | 0.51 | 0.00 | 0.00 |
| C16:1 | 0.06 | 0.06 | 0.28 | 0.28 | 0.07 | 0.07 | 0.50 | 0.29 | 0.25 | 0.12 | 0.00 | 0.00 |
| C16 | 55.57 | 25.53 | 240.49 | 63.68 | 101.18 | 22.27 | 263.90 | 54.47 | 176.60 | 33.99 | 3.99 | 1.49 |
| C18:1 | 0.64 | 0.35 | 1.87 | 0.64 | 0.92 | 0.14 | 1.19 | 0.75 | 1.17 | 0.22 | 0.00 | 0.00 |
| C18 | 6.21 | 3.04 | 28.88 | 9.89 | 12.97 | 3.15 | 36.23 | 10.41 | 16.44 | 3.91 | 0.54 | 0.54 |
| C20:1 | 0.01 | 0.01 | 0.10 | 0.10 | 0.01 | 0.01 | 0.07 | 0.07 | 0.04 | 0.04 | 0.00 | 0.00 |
| C20 | 0.31 | 0.14 | 1.61 | 0.42 | 0.94 | 0.15 | 2.68 | 0.38 | 0.87 | 0.10 | 0.00 | 0.00 |
| C22:1 | 0.31 | 0.12 | 1.34 | 0.49 | 0.35 | 0.35 | 1.47 | 0.74 | 0.66 | 0.20 | 0.00 | 0.00 |
| C22 | 3.08 | 1.17 | 17.18 | 3.59 | 8.34 | 1.95 | 25.20 | 4.28 | 8.62 | 1.52 | 0.24 | 0.24 |
| C24:1 | 34.72 | 11.85 | 203.00 | 54.79 | 115.16 | 30.89 | 320.84 | 78.76 | 103.84 | 21.41 | 5.83 | 2.08 |
| C24 | 19.11 | 7.16 | 89.54 | 14.31 | 42.22 | 6.60 | 118.84 | 16.18 | 57.39 | 5.01 | 1.80 | 0.55 |
Lipid extraction and purification were performed on 50 μl of each fraction. The sphingolipid composition of the fractions was then analyzed by UPLC-MS. The results are expressed in picomoles of lipids per milligram of proteins for each chain length of ceramide and SM (mean ± SEM). Cyto, cytosol; H, homogenate.