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. Author manuscript; available in PMC: 2021 Jan 3.
Published in final edited form as: J Proteome Res. 2020 Mar 4;19(4):1663–1673. doi: 10.1021/acs.jproteome.9b00843

Figure 5.

Figure 5.

Hat1 is required for the normal mitochondrial function. (A) Subset of mitochondrial proteins whose acetylation decreased at least 2.5-fold in Hat1 KO cells. (B) Cells were fractionated into nuclear, cytoplasmic, and mitochondrial extracts. Western blots of the extracts were probed with the indicated markers. VDAC is mitochondrial, GAPDH is cytoplasmic, and lamin is nuclear. Migration of molecular weight markers are indicated on the left. (C) Mitochondrial ones were purified by a percoll gradient. Western blot was probed with the indicated antibodies. Migration of molecular weight markers is indicated on the left (kDa). (D) HEK293 cells were transfected with the MitoLight expressing vector. Cells were visualized with a confocal microscope, and an individual Z-plane is shown.