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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Prog Retin Eye Res. 2019 Nov 6;76:100802. doi: 10.1016/j.preteyeres.2019.100802

Figure 4. 2DE analysis of human lens regions.

Figure 4.

Images of 2DE gels showing separation of proteins from a 42y human lens dissected into cortex (A), adult nucleus (B), fetal nucleus (C), and embryonic nucleus (D) regions. Figure from (Garland et al., 1996). Samples were solubilized in 9M urea, 2% NP40, 10mM DTT, 2% ampholytes (Resolyte pH 3.5–10). Proteins were separated based on isoelectric point using 18 cm non-linear, immobilized pH 3–10 gradients for 1st dimension isoelectric focusing (separation from acidic on the left to basic on the right) and based on size using SDS-PAGE in the second, vertical, dimension (18 × 25 cm gels). Each image represents total protein, water soluble and water insoluble, for all samples including the lens nucleus. Crystallin species migrated between pIs 8.6 and 5.