Table 2.

List of Crosslinked Proteins/Peptides Discovered in Human Lenses

Crosslinked proteins	Detected crosslinked peptide	Potential mechanism of crosslinking	Reference
βA4 crystallin C5-βA4 crystallin C166	βA4 2–7:acetyl-T LQC*TK βA4 159–174: GFQYVLEC* DHHSGDYK	DHA-mediated	Wang et al., 2014
βA4 crystallin C5-βA3 crystallin S59	βA4 2–7: acetyl-TLQC*TK βA3 46–64: MEFTSSCPNV SERS * FDNVR
βB2 crystallin K76-βB2 crystallin S204	βB2 69–81: GEQFVFEK* GEYPR βB2 199–205: GAFHPS*Da
βB1 crystallin S77-γS crystallin C130	βB1 74–86: AEFS*GECSNLADR βS 126–131: EIHSC*K
AQP0 N246-AQP0 K228	AQP0 239–263: GAKPDV SN*GQPEVTGEPVELDTQAL AQP0 227–233: LK*SISER	Succinimide	Friedrich et al., 2018
AQP0 N259-Lys	AQP0 239–263: GAKPDV SNGQPEVTGEPVELN * TQAL Unknown (maybe αB): K
βB1 crystallin K118-Spectrin D1207	βB1 111–123: GEMFILEK*GEYPR Spectrin 1207–1210: D*LR
βB2 crystallin K76-Spectrin D1207	βB2 69–81: GEQFVFEK*GEYPR Spectrin 1207–1210: D*LR
αA crystallin D151-αB crystallin K150	αA 146–157: IQTGLD*ATHAER αB 150–157: K*QVSGPER
AQP0 D246-AQP0 K228	AQP0 227–233: LK*SISER AQP0 239–246: GAKPDVSD*	succinic anhydride	unpublished
AQP0 K238-AQP0 D246	AQP0 234–243: LSVLK*GAKPD AQP0 239–246:GAKPDVSD*
αA crystallin D101-αB crystallin K174	αA:HD* αB: EEKPAVTAAPK*K
αA crystallin D101-αB crystallin K150	αA:HD* αB: K*QVSGPER
AQP0 M1-AQP0 D243	AQP0 1–5: *MWELR AQP0 239–243: GAKPD*	succinic anhydride	Wang et al., 2019
AQP0 D243-AQP0 K228	AQP0 227–233: LK*SISER AQP0 239–243: GAKPD*
AQP0 D243-γS crystallin K14	AQP0 239–243: GAKPD* γS 8–19: ITFYEDK*NFQGR
αA crystallin D151-αB crystallin K150	αA: IQTGLD* αB:K*QVSGPER
αA crystallin K78-αA crystallin D58	αA:FVIFLDVK*HPSPEDLTVK αA: TVLD*
αB crystallin D129 - αB crystallin K90	αB: IPADVD* αB: HFSPEELK*VK
αB crystallin D129 - αB crystallin K82	αB: IPADVD* αB: VK*VLGDVIEVHGK
αB crystallin D129 - αB crystallin K121	αB: IPADVD* αB: K*YR
αA crystallin D151 - αB crystallin K82	αA: IQTGLD* αB: VK*VLGDVIEVHGK
αA crystallin D151 – γC or yD crystallin G2	αA: IQTGLD* γC or γD: *GK
Filensin K467-βA4 Q195 or βA3 Q214	Filensin: SPKEPETPTELYTK#ER βA4 or βA3: IQ#Q	transglutaminase	unpublished
Filensin Q245-Phakinin K342	Filensin: VELQAQ* TTTLEQAIK Phakinin: ALK*R
Filensin K12-Phakinin Q361	Filensin: K#EQYEHADEASR Phakinin: HWHDMELQ#NLGAVVGR
αA crystallin K99 -αB crystallin K150	αA: VQDDFVEIHGK#HNER αB: KQ#VSGPER
βB1 crystallin Q236-Filensin K451	βB1: Q*WHLEGSFPVLATEPPK Filensin: EK*VR
Phakinin Q329-Phakinin C65	Phakinin: VELHNTS CQV Q#SLQAETESLR Phakinin: #CIGGLGAR	??
γD crystallin E8-γDcrystallin K3	γD: HYE*CSSDHPNLQPYLSR γD: G(carbamyl)K*ITLYEDR	unknown	unpublished
αB crystallin E99 -αB crystallin K121	αB: VLGDVIE*VHGK αB: K*YR
βB3 crystallin E177- γS K154	βB3: GE*YR γS: QYLLDK*K

^abolded residues indicate an Asp residue resulting from deamidation of an Asn residue in the nascent protein sequence.