Fig. 2.

Loss of Hsp90 chaperones suppress the neurite growth defects in mec-15 lf mutants. (A) ALM-PN is absent in mec-15; mec-7 mutants (arrowhead) and restored in mec-15; mec-7; sti-1 and mec-15; mec-7; pph-5 triple mutants (arrows). (B) Schematic representation of ALM and PLM morphologies in wild-type and various mutants. sti-1(−) is used as an example of mec-15(−) suppressors. (C) The length of ALM-PN in various mutants. hsp-90(ok1333), hsp-1(ok1371) and hsp-110(gk533) alleles caused larval arrest and were examined at L2/L3 stages. daf-41(ok3052), sti-1(u1071) and pph-5(u1072) were examined at both L2/L3 and adult stages; sti-1(ok3354) and pph-5(ok3498) deletion alleles served as references. mec-15; mec-7 adult animals carrying transgenes expressing dsRNA against hsf-1 or cdc-37 in TRNs were examined; results are labeled as hsf-1(i) and cdc-37(i), respectively. (D) Premature termination of ALM-AN and PLM-AN (arrowheads) in mec-15 single mutants was rescued in mec-15; sti-1 and mec-15; pph-5 double mutants. Dashed line indicates the position of the vulva. (E) Shortening of PLM-PN (arrowhead) in mec-15 single mutants was rescued to its normal length (arrows) in the double mutants. (F) The anterior extent of PLM-AN growth in 1-day-old adults of various mutants grown at 25°C. Distances are given relative to the position of the vulva, so positive values indicate that the PLM-AN grew past the vulva towards the anterior. (G) PLM-PN length in wild type (WT) and various mutants. For F and G, daf-41(ok3052), sti-1(u1071), pph-5(u1072), hsp-1(ok1371), hsp-90(ok1333) and hsp-110(gk533) were examined. Asterisks indicate statistical significance for the difference between the mec-7; mec-15 and the triple mutants (C) or between the mec-15 and the double mutants (G) in ANOVA and Tukey–Kramer test. Data are mean±s.d. (*P<0.05 and **P<0.01).