Fig. 7.
Behavior of hemichannels formed by Cx37-S319D and -S319A differ. (A) Representative traces show that Cx37-S319A HChs spend more time in open states than either Cx37-S319D or Cx37-WT HChs. The size of transitions is shown on the left of each recording; all-points histograms, on the right, show the frequency of each conductance state for the illustrated trace. (B) Transition frequency (mean±s.e.m. for each conductance bin) and open probability (Po) for HChs formed by Cx37-S319D and Cx37-S319A. Transitions of multiple amplitudes between fully open (∼750–800 pS) and closed were observed for both isoforms, but their open probabilities differed. Cx37-S319D preferred the closed state and a 60–90 pS subconductance state, whereas Cx37-S319A HChs preferred open states. Cx37-S319A (n=32, event no.=240) and Cx37-S319D (n=35, event no.=1521).