Fig. 4.
RA signaling inhibits the Wnt pathway to promote β cell specification and inhibit the δ cell transcriptional program. (A) Volcano plot of differentially expressed genes from whole-transcriptome analysis by RNA-seq of RARdnflox/flox; Neurog3:cre mutants (n=3 biological replicates). (B) A selection of genes from A with Log2 fold change and P-adjusted value (Padj≤0.05 is significant). (C) A selection of upregulated δ cell genes compared with Ins1/2 (Crhr2, Padj=0.053). (D) Significantly upregulated GO terms via PANTHER analysis of genes reported in A and Table S1. (E) Significantly enriched Wnt signaling components (GO:0017147). (F) Model demonstrating that during endocrine differentiation, RA signaling represses Wnt to promote β cell differentiation and repress δ-cell genes.