Fig. 5.

Overexpression of Prp8^S>F and Prp8^H>R induces apoptosis in developing eye primordium. (A–F) Compared with control (A) and prp8^del14/+ heterozygous EADs (B), ey-specific overexpression of Prp8^H>R (E) and Prp8^S>F (F) but not Prp8^wt (C) and Prp8^F>L (D) induces apoptosis as shown by immunostaining against the Drosophila Death caspase 1 (Dcp-1). The majority of apoptotic cells localize anterior to the morphogenetic furrow outside of the differentiating ELAV-positive photoreceptor clusters. (G–I) Co-expression of p35 reduces the amount of Dcp-1 positive cells in Prp8^H>R- and Prp8^S>F-expressing prp8^del14/+ heterozygous EADs (H,I) to levels observed in control (G). Representative micrographs are projections of multiple confocal sections showing EADs 7 days AEL. EAD outlines were generated based on DAPI signal. Scale bars: 50 µm.