Fig. 7.

Differentiated as well as uncommitted cells induce cytoprotective response to the Prp8^S>F and Prp8^H>R mutant variants. (A–F″) Ey-specific overexpression of Prp8^H>R (E) and Prp8^S>F (F) markedly induced the GstD1-GFP reporter activity in EADs of prp8^del14/+ heterozygous larvae compared to the background levels in controls (A,B) and EADs expressing Prp8^wt (C) and Prp8^F>L (D). Note the enhancement of the GstD1-GFP signal in both differentiating cells posterior as well as uncommitted epithelial cells anterior to the morphogenetic furrow expressing Prp8^H>R (E″) and Prp8^S>F (F″). Morphogenetic furrow and differentiated ommatidia clusters are visualized by immunostaining against p120-catenin. (G–J″) The GstD1-GFP induction in Prp8^H>R- and Prp8^S>F-expressing prp8^del14/+ heterozygous EADs (I″,J″) was not inhibited by co-expression of p35. Note that ey-driven p35 expression did not interfere with the endogenous GstD1-GFP reporter activity in the antenna (G″–J″). Representative micrographs are projections of multiple confocal sections showing EADs 7 days AEL. Images were acquired with the same intensity settings. Disc outlines were generated based on DAPI signal. Scale bars: 50 µm.