Fig. 8.

Spd-2 is required to recruit Cnn and the mitotic PCM to SAPs lacking Asl. (A) Confocal images of SAPs in 0- to 3-h-old eggs laid by females mutant for both asl^B46 and Spd-2. The eggs were stained for Asl (magenta), Sas-4 (red), GFP (SAPs, green) and α-Tubulin (blue) (left five panels), or Spd-2 (red), GFP (SAPs, green) and Cnn (blue) (right four panels). Note that, again, we often detected some staining in the Asl (far-red) channel in the SAPs formed in these double mutant eggs that we believe is probably bleed-through from the very intense Sas-4 (red) channel. (B) Number (left) and size (right) of the SAPs formed in eggs laid by females mutant for both asl^B46 and Spd-2. Each data point represents the average SAP size in an individual egg (N=6–156 SAPs per egg; n=62–103 eggs per genotype). The data were not all normally distributed so a Kruskal–Wallis test was used to assess statistical significance. (C) Sas-4 fluorescence signal intensity of SAPs (normalised to the SAP's GFP signal) in eggs laid by females mutant for both asl and Spd-2. The data was not all normally distributed so a Kruskal–Wallis test was used to assess statistical significance. (D) Percentage of SAPs in eggs laid by females mutant for both asl^B46 and Spd-2 that recruit detectable levels of Cnn, Spd-2, Sas-4 or α-Tubulin, as indicated. (E) Spd-2, Cnn and α-Tubulin fluorescence signal intensity of SAPs in eggs laid by females of the indicated genotypes. Error bars in B,C,E indicate s.d. ****P<0.0001.