Fig. 6.

OPTN BioID reveals novel partners and proteins localised to foci. (A) Graphs depicting significance analysis of interactome (SAINT) probability and fold change (FC-B) scores for BirA*–OPTN (top) and OPTN–BirA* (bottom) pulldown experiments. Selected high-confidence OPTN interactors are labelled. (B) Network diagram of high-confidence OPTN interactors identified by BioID. Node size corresponds to FC-B score (higher confidence=larger node). Solid lines indicate interactions identified in this study, and dashed lines interactions imported from publicly available protein–protein interaction databases. (C) Confocal microscopy images of RPE cells stably expressing GFP–OPTN E50K (green) and immunostained with an anti-p-TBK11 (i; red) or anti-HA antibody (ii–vi; red). Insets show magnified views of regions indicated by dashed boxes. Scale bar: 20 µm.