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. 2020 Jun 15;133(12):jcs239822. doi: 10.1242/jcs.239822

Fig. 8.

Fig. 8.

OPTN mutations regulate innate immune signalling and cytokine secretion. (A) Relative NF-κB luciferase activity in RPE cells expressing an NF-κB luciferase reporter alone (-), or with coexpression of GFP–OPTN E50K and E478G, and stimulated with poly(I:C) as indicated. Graph depicts mean±s.e.m. of n=6 independent experiments. *P<0.05; **P<0.01 (one-way ANOVA and a Bonferroni post-hoc test). (B,C) CXCL8 (B) and IL6 (C) secretion from RPE cells expressing GFP–OPTN E50K and E478G and stimulated with poly(I:C) as indicated. Graphs depict mean±s.e.m. of n=6 independent experiments. **P<0.01; ****P<0.0001 (one-way ANOVA and a Bonferroni post-hoc test). (D) Western blots of lysates from RPE cells expressing GFP–OPTN E50K or E478G, stimulated with poly(I:C) and probed with the indicated antibodies. (E) IFNα/β secretory levels from RPE cells coexpressing GFP–OPTN E50K and E478G and stimulated with poly(I:C) for 6 h as determined from luciferase activity induced in the ISRE-reporter cell line 3C11. Graph depicts mean±s.e.m. of n=5 independent experiments. ***P<0.001 (one-way ANOVA and a Bonferroni post-hoc test).