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. 2020 Jun 19;147(12):dev188987. doi: 10.1242/dev.188987

Fig. 1.

Fig. 1.

Generation of Tg(7xStat3-Hsv.Ul23:EGFP) fish and characterization of their expression pattern. (A) Scheme of the Tol-2 vectors used to generate the Tg(7xStat3-Hsv.Ul23:EGFP) reporter. (B,B′) Diffused EGFP is detectable in early stage embryos obtained by outcrossing transgenic females (B), not with transgenic males (B′). (C-F) At 22 hpf, EGFP expression is detectable in the anterior telencephalic region (t), the primordial midbrain hindbrain boundary (mhb), the hindbrain (h), the primitive neuromasts (n) and in the haematopoietic tissue (ht). (G,H) At 48 hpf, EGFP expression is mostly located in the optic tectum (TeO) and in the hindbrain (h). (I,J) Starting from 4 dpf, EGFP expression is detectable in the developing intestine in isolated pear-shaped cells (I); the intestinal fluorescence lasts throughout adulthood (J).