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. 2020 Jun 19;147(12):dev188987. doi: 10.1242/dev.188987

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Stat3 pathway is active in rapidly proliferating intestinal cells during zebrafish larval development. (A-A″) Co-localization (A″) using α-EGFP Ab (A) and EdU proliferation assay (A′) in the developing intestine (dashed line) of 5 dpf Tg(7xStat3:EGFP) larvae. (B,B′) Label retention assay using double transgenic Tg(7xStat3:EGFP)/Tg(HSP70:H2B:mRFP) 5 dpf larvae. The ubiquitous RFP label, accumulated in 100% of intestinal cells at 6 hpHS, is completely lost in EGFP-expressing cells at 48 hpHS, where no EGFP/RFP co-localization is appreciable (B′). (B″) RFP loss by Tg(7xStat3:EGFP)-positive intestinal cells. Three independent biological replicates were performed.