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. 2020 Jun 19;147(12):dev188987. doi: 10.1242/dev.188987

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Stat3 is required for intestinal homeostasis and larvae survival during development. (A) Percentage of the different genotypes found at different time points in stat3^+/− incross; stat3^−/− mutants were never found after 25 dpf, with the exception of a single animal that died at 51 dpf (shown in B). Statistical analysis was performed by chi-square test to compare proportions. (B) Haematoxylin-eosin staining of longitudinal sections at different larval stages (indicated on the left); arrowhead indicates the intestinal epithelium. Compared with normal fish, mutants display flattening of the intestinal epithelium. (C) Percentage of individuals at different stages presenting either normal intestine or degenerated intestine lacking intestinal folds. *P<0.05; **P<0.01.