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. 2020 Jun 17;147(12):dev187815. doi: 10.1242/dev.187815

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — CCR1 and ACKR2 are expressed surrounding epithelium in the mammary gland. (A) Flow cytometry analysis of CCR1 expression by enzymatically digested wild-type (black bars, n=6) and Ccr1^−/− (white bars, n=4) mammary gland cells: CD45+ SiglecF+, CD45+SiglecF-CD11b+F480+ and CD45−. (B) RNAscope in situ hybridisation of CCR1 (highlighted by a black arrow) and ACKR2 (highlighted by a red arrow) in the developing virgin mammary gland of WT, Ccr1^−/− and Ackr2^−/− mice. Significantly different results are indicated: two-tailed t-test, *P=0.0305. Data are mean±s.e.m.