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. 2020 Jun 17;147(12):dev187815. doi: 10.1242/dev.187815

Fig. 4.

Fig. 4.

Oestrogen induces CCR1 expression on macrophages. (A) Oestradiol levels in plasma from 5.5-week-old wild type (n=5), 6.5-week-old wild type (n=5), 7-week-old wild type (n=5) and 6.5-week-old Ackr2−/− (n=4). Two-tailed t-test (**P=0.0064). (B) CCR1 expression in CD11b+F4/80+ cells in response to DMSO and 50 µg/ml oestradiol in wild type (n=5), Ackr2−/− (n=3) and Ccr1−/− (n=4). Wild type, two-tailed t-test (**P=0.004); Ackr2−/−, two-tailed t-test (*P=0.0194). (C) Unsorted (n=3) and CD11b+F4/80+ FACS-sorted cells (n=2) from the mammary gland. Two-tailed t-test (*P=0.0217). (D) Transcription of CCR1 by CD11b+F4/80+ FACS-sorted cells from wild-type (n=4) and Ccr1−/− (n=2) mice, in response to DMSO or oestradiol. Two-tailed t-test (*P=0.0494). (E) CCR1 expression in CD11b+F4/80+ cells from female wild-type mammary glands at 4, 8 and 10 weeks of age, and in female 7-week-old Ccr1−/− mice and male wild-type inguinal fat pads from 7-week-old mice, in response to DMSO and 50 µg/ml 17β-oestradiol (each group, n=3). Two-tailed t-test (*P=0.04). (F) CCR1 expression in CD11b+F4/80+ cells from the peritoneum: wild type (n=5), Ackr2−/− (n=3) and Ccr1−/− (n=4). Significantly different results are indicated. Data are mean±s.e.m.