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. 2020 Jun 22;133(12):jcs242909. doi: 10.1242/jcs.242909

Fig. 4.

Fig. 4.

Active α5β1 integrin adhesions respond to changes in stiffness. (A) Representative images of TIFs plated on uniform stiffness hydrogels (0.8 kPa or 60 kPa) and stained for active α5β1-integrin and phospho-paxillin. Scale bars: 20 µm. (B) Tukey box plot of α5β1-integrin adhesion length (µm) from A [n=2 independent experiments, 9 cells (0.8 kPa) and 10 cells (60 kPa) analysed per experiment; 199 adhesions (0.8 kPa) and 211 adhesions (60 kPa)]. ***P<0.001. (C) Correlation analysis between substrate elastic modulus (kPa) and α5β1-integrin adhesion length (µm) in TIFs plated on a stiffness gradient hydrogel (0.5 kPa to 22 kPa) (n=1 independent experiment, 22 cells, >20 adhesions per cell). Error bars on the y-axis correspond to the s.d. on the adhesion length while error bars on the x-axis display error (95% c.i.) from the calibration curve used to convert from bead density into stiffness. (D) Representative images of TIFs stained for active α5β1-integrin from C across the stiffness gradient. Scale bars: 20 µm.