Skip to main content
. 2020 Jun 22;133(12):jcs242909. doi: 10.1242/jcs.242909

Fig. 5.

Fig. 5.

Tensins are required for adhesion elongation in response to stiffness. (A) Taqman qPCR analysis of tensin-1 silencing efficiency in TIF cells (n=4). Plots represent the normalized average value of RQ with its corresponding RQ min and RQ max. (B,C) TIF cells transfected with siRNA against tensin-1 (siTensin-1) and control-silenced TIFs were plated on stiffness gradient hydrogels and stained for active α5β1-integrin. (B) Comparison of active α5β1-integrin adhesion length across a hydrogel stiffness gradient between tensin-1-silenced TIFs (n=3 hydrogels) and controls (n=2); 20 cells were used per repeat with >20 adhesions per cell. Error bars on the y-axis correspond to the s.d. on the adhesion length while error bars on the x-axis display error (95% c.i.) from the calibration curve used to convert from bead density into stiffness. (C) Example images across the stiffness gradient are shown. Yellow squares denote magnified regions of interest of α5β1-integrin adhesions. Scale bars: 20 µm.