Figure 2.

Ablation of ghrelin exacerbated fasting-induced muscle atrophy. Changes of body weight, muscle mass and genes in 20-month-old wild-type (WT) and Ghrl^–/– mice after 48-hour fasting. (A) Body weight and (B) body weight change of WT and Ghrl^–/– mice. (C) Percentage of TA depot normalized to body weight. (D) Percentage of GM depot, n = 9. (E) Relative MyoD, myogenin, Atrogin-1, and MuRF-1 in GM, n = 4–5. (F) Western blots probed for MyoD, Myogenin, and GAPDH proteins, n = 5. Data are presented as mean ± SEM. *p < .05 versus WT mice, Student’s unpaired t test.