Skip to main content
. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res. 2020 May 18;44(6):1329–1336. doi: 10.1111/acer.14348

Fig. 3. Effect of gestational chronic binge alcohol exposure on mTORC1 signaling in the fetal hippocampus.

Fig. 3.

Immunoblot analysis showed that gestational chronic binge alcohol exposure significantly increased (A) P-4E-BP1 level (↑ 41%; P = 0.0156) and (B) total 4E-BP1 expression (↑ 20%; P = 0.0251) in the fetal hippocampi. (C) Immunofluorescence staining shows that P-4E-BP1 was expressed in the oriens layer (OL) of the fetal hippocampus and the representative image shows CA3 field near fimbria (FI). (D) Gestational alcohol exposure significantly increased (↑ 57%; P = 0.0383) the level of P-p70 S6K, whereas (E) total p70 S6K expression was not different between groups. Data are shown as mean ± SEM and as fold of control. *, Significance was established a priori at P < 0.05.