Cocaine injected female mice display elevated levels of G-CSF and G-CSF receptor. (A) Timeline of cocaine or saline injections followed by tissue collection and molecular analysis. (B) Cocaine treatment increased serum levels of G-CSF in female mice (saline, n = 8; cocaine, n = 10). (C,D) Cocaine treatment increased transcript levels of G-CSF (Csf 3: saline, n = 8; cocaine, n = 9) and receptor (Csf 3r: saline, n = 6; cocaine: n = 8) in the NAc. (E,F) Transcript levels of M-CSF (Csf1: saline, n = 9; cocaine, n = 9) and receptor (Csf1r: saline, n = 7; cocaine, n = 9) are unaffected by cocaine in the NAc. *p < 0.05, **p ≤ 0.01. All data are presented as mean ± SEM.