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. 2020 Jul 1;202(1):100–111. doi: 10.1164/rccm.201906-1232OC

Figure 5.

Figure 5.

Nanoparticle delivery of FOXM1 (forkhead box M1) or FOXF1 (forkhead box F1) improves alveolar microvascular network in hyperoxia (HO)-treated mice. (A) Immunostaining for endomucin shows alveolar microvascular networks (green) in P28 lungs. Mice were exposed to HO or room air (RA) from Postnatal Day 1 (P1) to P7, followed by RA exposure until lung harvest at P28. Nanoparticles containing cytomegalovirus (CMV)-Foxm1, CMV-Foxf1, or CMV-empty (control) were delivered at P2. DAPI was used to stain cell nuclei. The alveolar microvascular network is improved after nanoparticle delivery of FOXM1 or FOXF1. Scale bars: top panels, 50 μm; bottom panels, 10 μm. (B) Nanoparticle delivery of FOXM1 or FOXF1 increases capillary density in HO-injured lungs. Isolectin B4 was injected i.v. 1 hour before harvesting the mice at P14 (n = 3–4 mice per each group). (C and D) Endomucin-stained area (C) and isolectin B4–labeled volume (D) were quantified using 10 random lung images (n = 3–6 mice per group). Error bars are mean ± SD. **P < 0.01 and ***P < 0.001. n.s. = not significant.