Figure 2.

Screenshots from integrated genome viewer illustrating the compound in cis mutations ALK L1196M and G1202R detected from the patient at PD from lorlatinib therapy. Left panel is a zoom-out view that illustrates the sequencing reads for the region; the right panel is a base-level view that illustrates the base change. Colors represent the base change, wherein red represents the nucleotide threonine (T). The red column on the left represents reads with c.3604G>A (p.G1202R), while the red column on the right represents reads with c.3586C>A (p.L1196M), indicating substitution of C or G with A on the antisense strand. Exon 23 of ALK is depicted at the bottom left panel as a black box with repeating less-than symbols (<) indicating its antisense direction. Each gray row represents the sequencing read from a DNA fragment and the presence of both red bars on the same gray row represents their detection on the same strand of DNA. Two bars located at the bottom respectively indicate the nucleotide and amino acid sequence annotation of ALK.

ALK, anaplastic lymphoma kinase; PD, progressive disease.