Figure 4.

Kynurenine and TEACOPS do not significantly regulate IDO protein or activity. (a) Representative western blot and quantified densitometry of MSCs pre-licensed with increasing doses of kynurenine with or without heating. IDO protein was normalized to the house keeping gene β-actin, and further normalized to 0 μM kynurenine condition. N = 3 independent experiments. Reporting mean ± s.e.m. (b) Activity of IDO, measured as kynurenine concentration at the end of pre-licensing. Kynurenine produced was calculated by subtracting out signal generated due to kynurenine being added at the beginning of the experiment as measured on an identical plate without MSCs. N = 4 independent experiments. Reporting mean ± s.e.m.