Figure 5.

Dose, duration and donor are regulators of MSC immune modulation. (a) Proliferation of PBMCs co-cultured with MSC donors MSC1, MSC2 and MSC3 at a 1 : 10 PBMC to MSC ratio. %Proliferation was calculated by normalizing to stimulated PBMC control within each experiment (positive control). Reporting mean ± s.d. of three replicates for each donor. (b) Proliferation data from (a) pooled and replotted to compare the effects of pre-licensing strategies independent of donor. Each bar is a pre-licensing strategy and each data point is the mean %Proliferation for an MSC donor in that strategy. N = 3 independent donors, reporting mean ± s.e.m., two-way ANOVA with Dunnett's multiple comparisons test, *p < 0.05 considered significant. (c) Representative flow plots of CFSE-stained PBMCs upon co-culture with each MSC donor under each pre-licensing condition. Red line denotes gate used to calculate %PBMC proliferation reported in (a,b).