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. 2020 Jul;12(7):a035626. doi: 10.1101/cshperspect.a035626

Figure 2.

Figure 2.

Organogenesis of the liver and pancreas. (A–D) Schematic representation of the developing hepatopancreatic organ systems in the mouse embryos at the indicated stages. The liver grows dramatically during this developmental time window, forms characteristic hepatic chords, and invades the surrounding septum transversum mesenchyme. Dorsal and ventral pancreatic organ domains form epithelial buds and expand into the surrounding mesenchyme. The ventral pancreatic organ domain (A–C) eventually gives rise to gallbladder, common duct, and ventral pancreas (D). (A′–D′, A″–D″) Representative immunofluorescence stainings of the hepatopancreatic organ system at E9.0 (A′, A″), E9.5 (B′, B″), E10.0 (C′, C″), or E11.5 (D′, D″) either in wholemount (A′–D′) or on cryosections (A″–D″). Prox1 (red) marks all hepatopancreatic tissues, Pdx1 (blue) marks ventral and dorsal pancreas, and Sox17 (green) marks ventral pancreas, gallbladder, and endothelial cells. Staining for Ecad (red, D′) defines all epithelial tissues. (cd) common duct, (da) dorsal aorta, (dp) dorsal pancreas, (gb) gallbladder, (ht) heart, (lv) liver, (splm) splanchnic mesoderm, (stm) septum transversum mesenchyme, (vp) ventral pancreas. Scale bars, 100 µm.